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The effect of novel nitrogen-rich plasma polymer coatings on the phenotypic profile of notochordal cells

Fackson Mwale, Hong Wang, Alain Petit, Pierre-Luc Girard-Lauriault, Christopher J. Hunter, Jean A. Ouellet, Michael R. Wertheimer and John Antoniou

Article (2007)

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Cite this document: Mwale, F., Wang, H., Petit, A., Girard-Lauriault, P.-L., Hunter, C. J., Ouellet, J. A., ... Antoniou, J. (2007). The effect of novel nitrogen-rich plasma polymer coatings on the phenotypic profile of notochordal cells. BioMedical Engineering OnLine, 6, p. 33. doi:10.1186/1475-925x-6-33
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Abstract

Background: The loss of the notochordal cells from the nucleus pulposus is associated with ageing and disc degeneration. However, understanding the mechanisms responsible for the loss of these cells has been hampered in part due to the difficulty of culturing and maintaining their phenotype. Furthermore, little is known about the influence of the substratum on the molecular markers of notochordal cells. Methods: Notochordal cells were isolated from lumbar spine of non-chondrodystrophoid dogs and cultured on N-rich plasma polymer layers, so-called "PPE:N" ( N-doped plasma-polymerised ethylene, containing up to 36% [N]) surfaces, for 3, 7 or 14 days. Gene expression of vimentin ( VIM), pleiotrophin ( PTN), matrix Gla protein ( MGP), cartilage oligomeric matrix protein ( COMP), keratin 18 ( KRT 18), aggrecan ( AGG), collagen type 1 ( COL1A2), collagen type 2 ( COL2A1) was analyzed through semi-quantitative reverse transcription-polymerase chain reaction ( RT-PCR).Results: Notochordal cells were maintained in culture on PPE:N for up to 14 days with no loss in cell viability. Except for VIM, gene expression varied depending on the culture periods and [ N] concentration of the substratum. Generally, PPE: N surfaces altered gene expression significantly when cells were cultured for 3 or 7 days.Conclusion: The present study has shown that notochordal cells from dogs can attach to and grow on PPE: N surfaces. Analysis of the expression of different genes in these cells cultured on different N-functionalized surfaces indicates that cellular behaviour is gene-specific and time-dependent. Further studies are required to better understand the roles of specific surface functionalities on receptor sites, and their effects on cellular phenotypes.

Uncontrolled Keywords

Medicine (General); Medicine; Health Sciences; Medical technology; Medicine (General); Research; Plasma; Notochord; Animals; Dogs; Humans; Nitrogen; Ethylenes; Polymers; Biocompatible Materials; Biomedical Engineering; Gene Expression Regulation; Phenotype

Open Access document in PolyPublie
Subjects: 3100 Physique > 3100 Physique
3100 Physique > 3107 Physique des plasmas
Department: Département de génie physique
Research Center: Non applicable
Funders: Canadian Institutes for Health Research (CIHR), AO Foundation, Switzerland, CRSNG/NSERC
Date Deposited: 11 Jan 2019 17:10
Last Modified: 12 Jan 2019 01:20
PolyPublie URL: https://publications.polymtl.ca/3387/
Document issued by the official publisher
Journal Title: BioMedical Engineering OnLine (vol. 6)
Publisher: BioMed Central
Official URL: https://doi.org/10.1186/1475-925x-6-33

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