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High-throughput refractive index-based microphotonic sensor for enhanced cellular discrimination

Antoine Leblanc-Hotte, Geneviève Chabot-Roy, Livia Odagiu, Manon Richaud, Sylvie Lesage, Jean-Sébastien Delisle, Yves-Alain Peter

Article (2018)

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Abstract

This paper presents a novel microphotonic sensor based on silicon technologies for high-throughput single cell measurements. It employs a highly sensitive Fabry-Pérot resonant cavity to extract cellular refractive index information. The integrated large cross-section rib waveguides provide a single-mode like behavior important for resonant cell sensing. Differentiated myeloid cells derived from a promyelocytic leukemia cell line were injected in a microchannel, sheathlessly focused using inertial forces and analyzed while flowing through the resonant cavity volume. Results were compared against a commercial flow cytometer and showed a substantial improvement on cellular discrimination. Thus, this sensor has the ability to discriminate cell populations, usually identified using fluorescent parameters, without any dyes and can reach measurement rate as high as 2000 cells per second. By harnessing the cell's effective volume refractive index, our device offers complementary measurements readily improving actual technologies and thus providing crucial information for research and clinical professionals.

Uncontrolled Keywords

Microphotonic; Fabry-Pérot; Optical microcavity; Refractive index; Microfluidic; Cell sorting; Sensor

Subjects: 2500 Electrical and electronic engineering > 2505 Photonic devices and networks
3100 Physics > 3100 Physics
3100 Physics > 3110 Optics (see also Photon devices, 2505)
Department: Department of Engineering Physics
Funders: CRSNG/NSERC, Fonds de recherche du Québec - Nature et technologies (FRQNT)
Grant number: PGSD3-445848-2014, Equipe 173638, RQMP 187779
PolyPublie URL: https://publications.polymtl.ca/3024/
Journal Title: Sensors and Actuators B: Chemical (vol. 266)
Publisher: Elsevier
DOI: 10.1016/j.snb.2018.03.087
Official URL: https://doi.org/10.1016/j.snb.2018.03.087
Date Deposited: 17 Apr 2018 17:07
Last Modified: 17 Nov 2022 12:04
Cite in APA 7: Leblanc-Hotte, A., Chabot-Roy, G., Odagiu, L., Richaud, M., Lesage, S., Delisle, J.-S., & Peter, Y.-A. (2018). High-throughput refractive index-based microphotonic sensor for enhanced cellular discrimination. Sensors and Actuators B: Chemical, 266, 255-262. https://doi.org/10.1016/j.snb.2018.03.087

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