Antoine Leblanc-Hotte, Cindy Audiger, Geneviève Chabot-Roy, Félix Lombard-Vadnais, Jean-Sébastien Delisle, Yves-Alain Peter et Sylvie Lesage
Article de revue (2023)
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Abstract
Dendritic cells (DCs) patrol the organism at an immature stage to detect the presence of pathogens. Once activated, these mature DCs reach the lymph nodes to activate antigen-specific T lymphocytes and thus initiate an adaptative immune response to control the pathogen. The migration of both immature and mature DCs is a key process for their optimal function. DC migration requires transit through narrow constrictions that is allowed by their high local and global deformation capabilities. In addition to cytoplasmic changes, the nucleus mechanical properties also have a major impact for cellular migration and motility. Yet, nucleus intracellular mobility of dendritic cells or its variation upon maturation have not been investigated. Our study defines the biophysical phenotypic variations of dendritic cells upon maturation using interferometric deformability cytometry. This method characterizes different cellular mechanical properties, such as elongation and nucleus offset, by assessing the refractive index spatial distribution of shear-induced deformed cells. By using these parameters, our data suggest that in vitro bone marrow derived dendritic cell (BMDC) maturation induces cell stiffening and reduces nucleus mobility, allowing to distinguish immature and mature dendritic cells. Overall, our method provides insights on intracellular mechanical properties of two dendritic cell states.
Mots clés
Biological techniques; Biophysics; Biotechnology; Cell biology; Cell migration; Immunology; Lab-on-a-chip
Sujet(s): |
1900 Génie biomédical > 1900 Génie biomédical 5100 Biologie cellulaire > 5100 Biologie cellulaire 5100 Biologie cellulaire > 5109 Cibles intra-cellulaires 9000 Sciences de la santé > 9000 Sciences de la santé |
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Département: | Département de génie physique |
Organismes subventionnaires: | CRSNG/NSERC, Fonds de recherche du Québec - Nature et Technologies, Fondation de l’Hôpital Maisonneuve-Rosemont |
Numéro de subvention: | PGSD3-445848-2014, 173638, 255414, RQMP 187779 |
URL de PolyPublie: | https://publications.polymtl.ca/10798/ |
Titre de la revue: | Scientific Reports (vol. 13, no 1) |
Maison d'édition: | Nature Publishing Group |
DOI: | 10.1038/s41598-023-28625-w |
URL officielle: | https://doi.org/10.1038/s41598-023-28625-w |
Date du dépôt: | 07 févr. 2023 12:58 |
Dernière modification: | 28 sept. 2024 19:53 |
Citer en APA 7: | Leblanc-Hotte, A., Audiger, C., Chabot-Roy, G., Lombard-Vadnais, F., Delisle, J.-S., Peter, Y.-A., & Lesage, S. (2023). Immature and mature bone marrow-derived dendritic cells exhibit distinct intracellular mechanical properties. Scientific Reports, 13(1), 1967 (11 pages). https://doi.org/10.1038/s41598-023-28625-w |
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