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Immature and mature bone marrow-derived dendritic cells exhibit distinct intracellular mechanical properties

Antoine Leblanc-Hotte, Cindy Audiger, Geneviève Chabot-Roy, Félix Lombard-Vadnais, Jean-Sébastien Delisle, Yves-Alain Peter and Sylvie Lesage

Article (2023)

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Abstract

Dendritic cells (DCs) patrol the organism at an immature stage to detect the presence of pathogens. Once activated, these mature DCs reach the lymph nodes to activate antigen-specific T lymphocytes and thus initiate an adaptative immune response to control the pathogen. The migration of both immature and mature DCs is a key process for their optimal function. DC migration requires transit through narrow constrictions that is allowed by their high local and global deformation capabilities. In addition to cytoplasmic changes, the nucleus mechanical properties also have a major impact for cellular migration and motility. Yet, nucleus intracellular mobility of dendritic cells or its variation upon maturation have not been investigated. Our study defines the biophysical phenotypic variations of dendritic cells upon maturation using interferometric deformability cytometry. This method characterizes different cellular mechanical properties, such as elongation and nucleus offset, by assessing the refractive index spatial distribution of shear-induced deformed cells. By using these parameters, our data suggest that in vitro bone marrow derived dendritic cell (BMDC) maturation induces cell stiffening and reduces nucleus mobility, allowing to distinguish immature and mature dendritic cells. Overall, our method provides insights on intracellular mechanical properties of two dendritic cell states.

Uncontrolled Keywords

Biological techniques; Biophysics; Biotechnology; Cell biology; Cell migration; Immunology; Lab-on-a-chip

Subjects: 1900 Biomedical engineering > 1900 Biomedical engineering
5100 Cell biology > 5100 Cell biology
5100 Cell biology > 5109 Intracellular targeting
9000 Health sciences > 9000 Health sciences
Department: Department of Engineering Physics
Funders: CRSNG/NSERC, Fonds de recherche du Québec - Nature et Technologies, Fondation de l’Hôpital Maisonneuve-Rosemont
Grant number: PGSD3-445848-2014, 173638, 255414, RQMP 187779
PolyPublie URL: https://publications.polymtl.ca/10798/
Journal Title: Scientific Reports (vol. 13, no. 1)
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-023-28625-w
Official URL: https://doi.org/10.1038/s41598-023-28625-w
Date Deposited: 07 Feb 2023 12:58
Last Modified: 06 Apr 2024 21:52
Cite in APA 7: Leblanc-Hotte, A., Audiger, C., Chabot-Roy, G., Lombard-Vadnais, F., Delisle, J.-S., Peter, Y.-A., & Lesage, S. (2023). Immature and mature bone marrow-derived dendritic cells exhibit distinct intracellular mechanical properties. Scientific Reports, 13(1), 1967 (11 pages). https://doi.org/10.1038/s41598-023-28625-w

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