Manon Bardyn, Jérôme Allard, David Crettaz, Benjamin Rappaz, Gerardo Turcatti, Jean-Daniel Tissot et Michel Prudent
Article de revue (2021)
|
Libre accès au plein texte de ce document Version officielle de l'éditeur Conditions d'utilisation: Creative Commons: Attribution (CC BY) Télécharger (932kB) |
|
|
Libre accès au plein texte de ce document Matériel supplémentaire Conditions d'utilisation: Creative Commons: Attribution (CC BY) Télécharger (2MB) |
Abstract
An increase of oxygen saturation within blood bags and metabolic dysregulation occur during storage of red blood cells (RBCs). It leads to the gradual exhaustion of RBC antioxidant protective system and, consequently, to a deleterious state of oxidative stress that plays a major role in the apparition of the so-called storage lesions. The present study describes the use of a test (called TSOX) based on fluorescence and label-free morphology readouts to simply and quickly evaluate the oxidant and antioxidant properties of various compounds in controlled conditions. Here, TSOX was applied to RBCs treated with four antioxidants (ascorbic acid, uric acid, trolox and resveratrol) and three oxidants (AAPH, diamide and H2O2) at different concentrations. Two complementary readouts were chosen: first, where ROS generation was quantified using DCFH-DA fluorescent probe, and second, based on digital holographic microscopy that measures morphology alterations. All oxidants produced an increase of fluorescence, whereas H₂O₂ did not visibly impact the RBC morphology. Significant protection was observed in three out of four of the added molecules. Of note, resveratrol induced diamond-shape “Tirocytes”. The assay design was selected to be flexible, as well as compatible with high-throughput screening. In future experiments, the TSOX will serve to screen chemical libraries and probe molecules that could be added to the additive solution for RBCs storage.
Mots clés
red blood cell; transfusion; antioxidant; oxidant; high-throughput screening; digital holographic microscopy; fluorescence
Sujet(s): |
1800 Génie chimique > 1800 Génie chimique 1800 Génie chimique > 1802 Génie biochimique |
---|---|
Département: | Département de génie chimique |
Organismes subventionnaires: | CETRASA foundation |
URL de PolyPublie: | https://publications.polymtl.ca/9392/ |
Titre de la revue: | International Journal of Molecular Sciences (vol. 22, no 8) |
Maison d'édition: | MDPI |
DOI: | 10.3390/ijms22084293 |
URL officielle: | https://doi.org/10.3390/ijms22084293 |
Date du dépôt: | 09 févr. 2023 17:03 |
Dernière modification: | 16 août 2024 10:34 |
Citer en APA 7: | Bardyn, M., Allard, J., Crettaz, D., Rappaz, B., Turcatti, G., Tissot, J.-D., & Prudent, M. (2021). Image- and fluorescence-based test shows oxidant-dependent damages in red blood cells and enables screening of potential protective molecules. International Journal of Molecular Sciences, 22(8), 4293 (14 pages). https://doi.org/10.3390/ijms22084293 |
---|---|
Statistiques
Total des téléchargements à partir de PolyPublie
Téléchargements par année
Provenance des téléchargements
Dimensions