Data from: A multivalent mRNA-LNP vaccine protects against Clostridioides difficile infection

Alexa Semon, Mohamad-Gabriel Alameh, Nile Bayard, Yi-Gen Pan, James Knox, Garima Dwivedi, Rochelle Glover, Paula Rangel, Ceylan Tanes, Kyle Bittinger, Qianxuan She, Haitao Hu, Srinivasa Reddy Bonam, Jeffrey Maslanka, Paul Planet, Ahmed Moustafa, Benjamin Davis, Anik Chevrier, Mitchell Beattie, Houping Ni, Gabrielle Blizard, Emma Furth, Robert Mach, Marc Lavertu , Mark Sellmyer, Ying Tam, Michael C. Abt, Drew Weissman and Joseph Zackular

Dataset (2024)

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Abstract

Clostridioides difficile infection (CDI) is an urgent public health threat with limited therapeutic options. In this work, we developed a messenger RNA (mRNA)-lipid nanoparticle (LNP) vaccine targeting C. difficile toxins and virulence factors. This multivalent vaccine elicited robust and long-lived systemic and mucosal antigen-specific humoral and cellular immune responses across animal models and independent of changes to the intestinal microbiota. Vaccination protected mice from lethal CDI in both primary and recurrent infection models, and inclusion of non-toxin cellular and spore antigens improved decolonization of toxigenic C. difficile from the gastrointestinal tract. Our studies demonstrate mRNA-LNP vaccine technology as a promising platform for the development of novel C. difficile therapeutics with potential for limiting acute disease and promoting bacterial decolonization.

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Supplementary Material:	Article
Department:	Department of Chemical Engineering
Funders:	NIH, NIAID
Grant number:	R01AI158830, 5U19AI174998-03
PolyPublie URL:	https://publications.polymtl.ca/62651/
Source:	Dryad
DOI:	10.5061/dryad.98sf7m0sg
Official URL:	https://doi.org/10.5061/dryad.98sf7m0sg
Date Deposited:	10 Feb 2025 17:32
Last Modified:	27 Jan 2026 15:41

Cite in APA 7:	Semon, A., Alameh, M.-G., Bayard, N., Pan, Y.-G., Knox, J., Dwivedi, G., Glover, R., Rangel, P., Tanes, C., Bittinger, K., She, Q., Hu, H., Bonam, S. R., Maslanka, J., Planet, P., Moustafa, A., Davis, B., Chevrier, A., Beattie, M., ... Zackular, J. (2024). Data from: A multivalent mRNA-LNP vaccine protects against Clostridioides difficile infection [Dataset]. Dryad. https://doi.org/10.5061/dryad.98sf7m0sg

Cite in APA 7:

Semon, A., Alameh, M.-G., Bayard, N., Pan, Y.-G., Knox, J., Dwivedi, G., Glover, R., Rangel, P., Tanes, C., Bittinger, K., She, Q., Hu, H., Bonam, S. R., Maslanka, J., Planet, P., Moustafa, A., Davis, B., Chevrier, A., Beattie, M., ... Zackular, J. (2024). Data from: A multivalent mRNA-LNP vaccine protects against Clostridioides difficile infection [Dataset]. Dryad. https://doi.org/10.5061/dryad.98sf7m0sg

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