Fackson Mwale, Michael R. Wertheimer et John Antoniou
Communication écrite (2008)
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Abstract
Degenerative disc disease has been implicated as a major component of spine pathology. However, though biological repair of the degenerate disc would be the ideal treatment, there is a lack of a universally accepted scaffold for tissue engineering of intervertebral discs (IVD) and little is known of how to differentiate mesenchymal stem cells (MSCs) to a disc-like phenotype. We show that 2.5% Protasan® UP G213 cross-linked to 5% genipin might be a promising scaffold for disc tissue engineering. Furthermore, we have developed extremely N-rich plasma polymer layers, which we call "PPE:N" (N-doped plasma-polymerized ethylene, containing up to 36% [N]). We show that PPE:N almost completely suppresses the expression not only of type X collagen, but also of osteogenic marker genes such as alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OC). In contrast, neither aggrecan nor types 1 collagen expression were significantly affected. These results indicate that PPE:N coatings may be suitable surfaces for inducing MSCs to a chondrocyte or disc-like phenotype for tissue engineering of cartilage or IVDs, in which hypertrophy and osteogenesis are suppressed.
Département: | Département de génie physique |
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URL de PolyPublie: | https://publications.polymtl.ca/55211/ |
Nom de la conférence: | 3rd International Conference on Smart Materials, Structures and Systems (CIMTEC 2008) |
Lieu de la conférence: | Sicily, Italy |
Date(s) de la conférence: | 2008-06-08 - 2008-06-13 |
Éditeurs ou éditrices: | Pietro Vincenzini et Danilo De Rossi |
Maison d'édition: | Trans Tech Publications |
DOI: | 10.4028/www.scientific.net/ast.57.117 |
URL officielle: | https://doi.org/10.4028/www.scientific.net/ast.57.... |
Date du dépôt: | 18 sept. 2023 09:19 |
Dernière modification: | 08 avr. 2024 19:34 |
Citer en APA 7: | Mwale, F., Wertheimer, M. R., & Antoniou, J. (juin 2008). Novel Nitrogen Rich Polymers and Chitosan for Tissue Engineering of Intervertebral Discs [Communication écrite]. 3rd International Conference on Smart Materials, Structures and Systems (CIMTEC 2008), Sicily, Italy (280 pages). https://doi.org/10.4028/www.scientific.net/ast.57.117 |
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