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Novel Nitrogen Rich Polymers and Chitosan for Tissue Engineering of Intervertebral Discs

Fackson Mwale, Michael R. Wertheimer and John Antoniou

Paper (2008)

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Abstract

Degenerative disc disease has been implicated as a major component of spine pathology. However, though biological repair of the degenerate disc would be the ideal treatment, there is a lack of a universally accepted scaffold for tissue engineering of intervertebral discs (IVD) and little is known of how to differentiate mesenchymal stem cells (MSCs) to a disc-like phenotype. We show that 2.5% Protasan® UP G213 cross-linked to 5% genipin might be a promising scaffold for disc tissue engineering. Furthermore, we have developed extremely N-rich plasma polymer layers, which we call "PPE:N" (N-doped plasma-polymerized ethylene, containing up to 36% [N]). We show that PPE:N almost completely suppresses the expression not only of type X collagen, but also of osteogenic marker genes such as alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OC). In contrast, neither aggrecan nor types 1 collagen expression were significantly affected. These results indicate that PPE:N coatings may be suitable surfaces for inducing MSCs to a chondrocyte or disc-like phenotype for tissue engineering of cartilage or IVDs, in which hypertrophy and osteogenesis are suppressed.

Department: Department of Engineering Physics
PolyPublie URL: https://publications.polymtl.ca/55211/
Conference Title: 3rd International Conference on Smart Materials, Structures and Systems (CIMTEC 2008)
Conference Location: Sicily, Italy
Conference Date(s): 2008-06-08 - 2008-06-13
Editors: Pietro Vincenzini and Danilo De Rossi
Publisher: Trans Tech Publications
DOI: 10.4028/www.scientific.net/ast.57.117
Official URL: https://doi.org/10.4028/www.scientific.net/ast.57....
Date Deposited: 18 Sep 2023 09:19
Last Modified: 08 Apr 2024 19:34
Cite in APA 7: Mwale, F., Wertheimer, M. R., & Antoniou, J. (2008, June). Novel Nitrogen Rich Polymers and Chitosan for Tissue Engineering of Intervertebral Discs [Paper]. 3rd International Conference on Smart Materials, Structures and Systems (CIMTEC 2008), Sicily, Italy (280 pages). https://doi.org/10.4028/www.scientific.net/ast.57.117

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