Marie Christine Uwamahoro, Richard Massicotte, Yves Hurtubise, François Gagné-Bourque, Akier Assanta Mafu et L'Hocine Yahia
Article de revue (2018)
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Abstract
Spore-forming pathogenic bacteria, such as Clostridium difficile, are associated with nosocomial infection, leading to the increased use of sporicidal disinfectants, which impacts socioeconomic costs. However, C. difficile can be prevented using microorganisms such as Bacillus amyloliquefaciens, a prophylactic agent that has been proven to be effective against it in recent tests or it can be controlled by sporicidal disinfectants. These disinfectants against spores should be evaluated according to a known and recommended standard. Unfortunately, some newly manufactured disinfectants like Bioxy products have not yet been tested. ASTM E2197-11 is a standard test that uses stainless steel disks (1 cm in diameter) as carriers, and the performance of the test formulation is calculated by comparing the number of viable test organisms to that on the control carriers. Surface tests are preferable for evaluating disinfectants with sporicidal effects on hard surfaces. This study applies improved methods, based on the ASTM E2197-11 standard, for evaluating and comparing the sporicidal efficacies of several disinfectants against spores of C. difficile and B. amyloliquefaciens, which are used as the test organisms. With the improved method, all spores were recovered through vortexing and membrane filtration. The results show that chlorine-based products are effective in 5 min and Bioxy products at 5% w/v are effective in 10 min. Although Bioxy products may take longer to prove their effectiveness, their non-harmful effects to hospital surfaces and people have been well established in the literature.
Mots clés
Bacillus amyloliquefaciens; Clostridium difficile; disinfectant; spores; sporicidal
Sujet(s): | 5200 Microbiologie > 5200 Microbiologie |
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Département: | Département de génie mécanique |
Centre de recherche: | LIAB - Laboratoire d'innovation et d'analyse de bioperformances |
Organismes subventionnaires: | Programme Canadien de bourses de la Francophonie (PCBF), Federal government of Canada engagement grant |
URL de PolyPublie: | https://publications.polymtl.ca/5157/ |
Titre de la revue: | Frontiers Public Health (vol. 6) |
Maison d'édition: | Frontiers |
DOI: | 10.3389/fpubh.2018.00018 |
URL officielle: | https://doi.org/10.3389/fpubh.2018.00018 |
Date du dépôt: | 05 juil. 2022 12:24 |
Dernière modification: | 28 sept. 2024 01:10 |
Citer en APA 7: | Uwamahoro, M. C., Massicotte, R., Hurtubise, Y., Gagné-Bourque, F., Mafu, A. A., & Yahia, L. (2018). Evaluating the sporicidal activity of disinfectants against Clostridium difficile and Bacillus amyloliquefaciens spores by using the improved methods based on ASTM E2197-11. Frontiers Public Health, 6, 18 (6 pages). https://doi.org/10.3389/fpubh.2018.00018 |
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