Reconfigurable microfluidic magnetic valve arrays: Towards a radiotherapy-compatible spheroid culture platform for the combinatorial screening of cancer therapies

Abstract

We introduce here a microfluidic cell culture platform or spheroid culture chamber array (SCCA) that can synthesize, culture, and enable fluorescence imaging of 3D cell aggregates (typically spheroids) directly on-chip while specifying the flow of reagents in each chamber via the use of an array of passive magnetic valves. The SCCA valves demonstrated sufficient resistance to burst (above 100 mBar), including after receiving radiotherapy (RT) doses of up to 8 Gy combined with standard 37 degrees C incubation for up to 7 days, enabling the simultaneous synthesis of multiple spheroids from different cell lines on the same array. Our results suggest that SCCA would be an asset in drug discovery processes, seeking to identify combinatorial treatments.

Uncontrolled Keywords

Cell Culture Techniques; Cell Line, Tumor; Humans; *Microfluidics; Neoplasms; Spheroids, Cellular; chemotherapy; drug discovery; microfluidics; radiotherapy; spheroids; valves