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Reconfigurable microfluidic magnetic valve arrays: Towards a radiotherapy-compatible spheroid culture platform for the combinatorial screening of cancer therapies

Alexandre R. Brunet, Frédérique Labelle, Philip Wong and Thomas Gervais

Article (2017)

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Cite this document: Brunet, A. R., Labelle, F., Wong, P. & Gervais, T. (2017). Reconfigurable microfluidic magnetic valve arrays: Towards a radiotherapy-compatible spheroid culture platform for the combinatorial screening of cancer therapies. Sensors, 17(10). doi:10.3390/s17102271
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Abstract

We introduce here a microfluidic cell culture platform or spheroid culture chamber array (SCCA) that can synthesize, culture, and enable fluorescence imaging of 3D cell aggregates (typically spheroids) directly on-chip while specifying the flow of reagents in each chamber via the use of an array of passive magnetic valves. The SCCA valves demonstrated sufficient resistance to burst (above 100 mBar), including after receiving radiotherapy (RT) doses of up to 8 Gy combined with standard 37 degrees C incubation for up to 7 days, enabling the simultaneous synthesis of multiple spheroids from different cell lines on the same array. Our results suggest that SCCA would be an asset in drug discovery processes, seeking to identify combinatorial treatments.

Uncontrolled Keywords

Cell Culture Techniques; Cell Line, Tumor; Humans; *Microfluidics; Neoplasms; Spheroids, Cellular; chemotherapy; drug discovery; microfluidics; radiotherapy; spheroids; valves

Open Access document in PolyPublie
Subjects: 1900 Génie biomédical > 1900 Génie biomédical
1900 Génie biomédical > 1901 Technologie biomédicale
1900 Génie biomédical > 1902 Matériaux biomédicaux
3100 Physique > 3100 Physique
3100 Physique > 3101 Études atomiques et moléculaires
3100 Physique > 3110 Optique (voir aussi Dispositifs photoniques, 2505)
Department: Département de génie physique
Institut de génie biomédical
Polytechnique Montréal > Centres de recherche > Institut de génie biomédical
Research Center: Non applicable
Funders: CRSNG/NSERC, Cancer Research Society, Canderel Scholarship, Fonds de Recherche Québec - Santé
Grant number: 20276
Date Deposited: 14 Jul 2021 14:53
Last Modified: 15 Jul 2021 01:20
PolyPublie URL: https://publications.polymtl.ca/4809/
Document issued by the official publisher
Journal Title: Sensors (vol. 17, no. 10)
Publisher: MDPI
Official URL: https://doi.org/10.3390/s17102271

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