Alexandre R. Brunet, Frédérique Labelle, Philip Wong and Thomas Gervais
Article (2017)
|
Published Version Terms of Use: Creative Commons Attribution . Download (2MB) |
Cite this document: | Brunet, A. R., Labelle, F., Wong, P. & Gervais, T. (2017). Reconfigurable microfluidic magnetic valve arrays: Towards a radiotherapy-compatible spheroid culture platform for the combinatorial screening of cancer therapies. Sensors, 17(10). doi:10.3390/s17102271 |
---|
Abstract
We introduce here a microfluidic cell culture platform or spheroid culture chamber array (SCCA) that can synthesize, culture, and enable fluorescence imaging of 3D cell aggregates (typically spheroids) directly on-chip while specifying the flow of reagents in each chamber via the use of an array of passive magnetic valves. The SCCA valves demonstrated sufficient resistance to burst (above 100 mBar), including after receiving radiotherapy (RT) doses of up to 8 Gy combined with standard 37 degrees C incubation for up to 7 days, enabling the simultaneous synthesis of multiple spheroids from different cell lines on the same array. Our results suggest that SCCA would be an asset in drug discovery processes, seeking to identify combinatorial treatments.
Uncontrolled Keywords
Cell Culture Techniques; Cell Line, Tumor; Humans; *Microfluidics; Neoplasms; Spheroids, Cellular; chemotherapy; drug discovery; microfluidics; radiotherapy; spheroids; valves
![]() |
|
Subjects: |
1900 Génie biomédical > 1900 Génie biomédical 1900 Génie biomédical > 1901 Technologie biomédicale 1900 Génie biomédical > 1902 Matériaux biomédicaux 3100 Physique > 3100 Physique 3100 Physique > 3101 Études atomiques et moléculaires 3100 Physique > 3110 Optique (voir aussi Dispositifs photoniques, 2505) |
---|---|
Department: |
Département de génie physique Institut de génie biomédical |
Research Center: | Non applicable |
Funders: | CRSNG/NSERC, Cancer Research Society, Canderel Scholarship, Fonds de Recherche Québec - Santé |
Grant number: | 20276 |
Date Deposited: | 14 Jul 2021 14:53 |
Last Modified: | 15 Jul 2021 01:20 |
PolyPublie URL: | https://publications.polymtl.ca/4809/ |
![]() |
|
Journal Title: | Sensors (vol. 17, no. 10) |
---|---|
Publisher: | MDPI |
Official URL: | https://doi.org/10.3390/s17102271 |
Statistics
Total downloads
Downloads per month in the last year
Origin of downloads
Dimensions